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Generation of pac fragments. (A) Cutting of concatemeric DNA starting from the pac site (○), with sequential cuts according to the headful mechanism. (B) Partial genomic map of PY100 showing the position of the putative pac site (boxed nucleotides) and selected restriction sites of enzymes generating the pac fragments (see also Fig. 4). The black box indicates the PCR probe (primers PY100-5 and PY100-6) that was used for hybridization.
