TABLE 2.

Selected genes of phage PY100^a

ORF	Gene	Start-stop	Protein size (aa)b	Potential function (gene)	Significant matches (source)
2	ORF2	491-1045	184	Terminase small subunit (terS)	Terminase small subunit (enterobacterium phage P22)
18	ORF18	6909-5440	489	Terminase large subunit (terL)	Putative terminase large subunit TerL (bacteriophage AaΦ23)
34	ORF34	12756-12109	215	Dam-methylase (dam)	Phage DNA methyltransferase (Sodalis glossinidius strain) (enterobacterium phage T1)
35	ORF35	13367-12804	187	Single-stranded DNA binding (ssb)	Single-stranded DNA-binding protein (Vibrio sp. strain MED222)
48	ORF48	17422-17838	127	Endolysine	Bacteriophage P7-related protein (Yersinia pseudotuberculosis IP 32953)
52	ORF52	18620-19810	396		Hypothetical protein T1p16 (enterobacterium phage T1)
53	ORF53	19854-20312	152	Polynucleotide kinase/phosphatase	Putative polynucleotide kinase/phosphatase (enterobacterium phage T1)
54	ORF54	20235-20912	195		Hypothetical protein T1p62 (enterobacterium phage)
55	ORF55	20979-22340	453	Portal protein	Hypothetical protein (XF1571 Xylella fastidiosa 9a5c) (T1p52 enterobacterium phage T1)
56	ORF56	22300-23133	277	Head protein	Putative bacteriophage protein (Acinetobacter sp. strain ADP1) (T1p51 enterobacterium phage T1)
57	ORF57	23145-24305	386	Head protein	Hypothetical protein (lin1728 Listeria innocua Clip11262) (T1p50 enterobacterium phage T1)
58	ORF58	24308-24796	162		Hypothetical protein lin1727 (Listeria innocua Clip11262)
59	ORF59	24886-25893	335	Head protein	Hypothetical protein (CGSHi22121_00927) (Haemophilus influenzae 22.1-21) (T1p47 enterobacterium phage T1)
63	ORF63	27035-27403	122
64	ORF64	27400-27930	176
65	ORF65	27975-29114	379		Bacteriophage Felix 01
66	ORF66	29118-29546	142		Hypothetical protein PD1187 (Xylella fastidiosa Temecula1)
70	ORF70	30763-32271	502	Tail tape measure protein	Phage-related protein (Xylella fastidiosa Ann-1)
71	ORF71	32271-32918	215		Putative bacteriophage protein (Ralstonia eutropha JMP134)
73	ORF73	33313-34380	355		Phage-related protein (Xylella fastidiosa Dixon)
74	ORF74	34358-34951	197	Baseplate protein	Conserved hypothetical protein (Xylella fastidiosa Dixon)
75	ORF75	34948-35304	118		Hypothetical protein CGSHi22121_00997 (Haemophilus influenzae 22.1-21)
76	ORF76	35315-36490	391		COG3299: uncharacterized homolog of phage Mu protein gp47 (Magnetospirillum magnetotacticum MS-1)
77	ORF77	36493-37158	221		Hypothetical protein plu2875 (Photorhabdus luminescens subsp. laumondii TTO1)
78	ORF78	37151-38206	351	Tail fiber protein	Hypothetical protein (Ecol5_01002866 Escherichia coli 53638)
79	ORF79	38179-38736	185	Tail fiber assembly protein	Putative bacteriophage protein (Yersinia pseudotuberculosis IP 32953)
81	ORF81	38887-40809	640	Tail fiber protein	Tail fiber protein (Yersinia phage PY54)
82	ORF82	40794-41225	143	Tail fiber assembly protein	Tail fiber assembly protein (Yersinia phage PY54)
83	ORF83	41268-42035	255	Tail protein	gp37 long tail fiber, distal subunit (enterobacterium phage T4)
86	ORF86	42787-44475	562	Replicative DNA helicase (dnaB)	Hypothetical protein PBPRB1439 (Photobacterium profundum SS9)
88	ORF88	44964-46682	572	NTP-dependent helicase (helA)	Putative ATP-dependent helicase (enterobacterium phage T1)
89	ORF89	46682-47185	167		Hypothetical protein T1p21 (enterobacterium phage T1)
90	ORF90	47215-48213	332	DNA recombination protein (recT)	Phage-related DNA recombination protein (Bordetella bronchiseptica RB50)
91	ORF91	48214-48702	162	HNH endonuclease (endC)	Bacteriophage Felix 01
92	ORF92	48702-49400	232	Exonuclease	Exonuclease (Escherichia coli O157:H7 strain Sakai)

^aORFs whose gene products were detected by MS are indicated in boldface type.

^baa, amino acids.