TABLE 4.
Strain | Construct | Spacinga | Genotype
|
LacZ sp act (arbitrary units)b
|
Repression (fold) | ||
---|---|---|---|---|---|---|---|
narL | narP | No NO3− | NO3− | ||||
VJS8886 | O3-lac O1-lac | 91 | + | + | 3,290 | 2,050 | 1.6 |
VJS9003 | O3-napF O1-lac | 91 | + | + | 2,680 | 2,210 | 1.2 |
VJS9002 | O3-napF O1-lac | 87 | + | + | 2,770 | 2,200 | 1.3 |
Interoperator spacing (see Fig. 1).
Strains were cultured overnight in MOPS defined medium with limiting glucose and 0.1 M IPTG.