TABLE 1.
Mapping the COS in selected promoters relative to their putative −35 and −10 boxes
| Open reading frame IDs for strains COL and N315 | Gene | Sequencea |
|---|---|---|
| SACOL2002, SA1751 | eapb | taatattcAGTTAATTcaaaaaatagagaaagtctggctataattaagtt N36 ATG |
| SACOL0858, SA0744 | empb | ttacttcaAGTTAATTattatactgaaaattctggtatataatgctgtta N63 ATG |
| SACOL0857, SA0743 | vwb | tcatcaacAGTTAATTattttaaaaaggtagatgttatataatttggctt N60 ATG |
| SACOL1168, SA1003 | efbb | gtgtttatAGTTAATTaataattAGTTAATTtcaaaagttgtataaataggataactt N36 ATG |
| SACOL2419, SA2207 | hlgAb | taacgaatAGTTAATTcgaaaacgcttacaaatggattattatatatatgaactta N27 ATG |
| SACOL2421, SA2208 | hlgCb | taatgaacAGTTAATTataataacgcccaaaatatattattat N41 ATG |
| SACOL2418, SA2206 | sbib | taataattAGTTAATTtccatttgtattctcatgtgataaatt N36 ATG |
| SACOL1881, SA1638 | lukEc | taatgaacAGTTAATTttcataaataagtgaaataatctagaataaatttg N34 ATG |
| SACOL1880, SA1637 | lukDc | agatataaAGTTAATTggaaaacacacgaaattaaagtgaaaggacataattaat ATG |
| SACOL1389, SA1300 | parE | atgaaAGTTAATTgatacttgcattttacgctgatttatat N63 ATG |
| SACOL2136, SA1946 | Hypothetical protein of czr operon | tcactaatAGTTAATTtgaaatgtggaagtttataatacgat N82 ATG |
a
The COS is shown in uppercase, putative −35 boxes are underlined and in italics, putative −10 boxes are underlined, and experimentally mapped transcription start points are in bold and underlined. Putative −35 and −10 boxes were identified by visual inspection of the respective promoters. The COS is also found on the complementary strand of the abcA (21) and icaR (8) promoters.
b
SaeRS-regulated gene (39).
c
lukE and lukD form a bicistronic operon. The COS in lukD is located just upstream of a ribosome binding site.