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. 2007 Oct 19;190(1):387–400. doi: 10.1128/JB.00765-07

FIG. 10.

FIG. 10.

PptA-mediated PilE modification in P. aeruginosa and E. coli. (A) MS analysis of intact PilE expressed in P. aeruginosa. (B) MS analysis of intact PilE coexpressed with PptA in P. aeruginosa. (C) Intact mass analysis of a PilE(S68A) mutant coexpressed with PptA in P. aeruginosa. (D) Immunoblot of whole-cell lysates using polyclonal antibodies specific for PilE. Lanes 1 to 3 (from left), P. aeruginosa pilA pilT expressing N. gonorrhoeae proteins: lane 1, PilE; lane 2, PilE(S68A); lane 3, PilE and PptA. Lanes 4 and 5, E. coli DH5α expressing the proteins: lane 4, PilE; lane 5, PilE and PptA. Peaks in the MS spectra that are not labeled are listed in Table S3 in the supplemental material.