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. 2007 Oct 19;190(1):387–400. doi: 10.1128/JB.00765-07

FIG. 4.

FIG. 4.

PilE PC modification levels parallel regulated expression of PptA. (A) Lane 1 (from left), KS9 (pptA::kan); lane 2, KS11 without IPTG [pilV(Fs pptA::kan iga::pptA(Ind)]. Lanes 3 to 9, KS11 with IPTG in the following concentrations: lane 3, 0.01 mM; lane 4, 0.015 mM; lane 5, 0.02 mM; lane 6, 0.03 mM; lane 7, 0.04 mM; lane 8, 0.05 mM; lane 9, 0.1 mM; lane 10, control (GV1). (B to D) Deconvoluted molecular weight spectra from intact PilE ESI MS analyses in strains KS11 without IPTG [pilV(Fs) pptA::kan iga::pptA(Ind)] (B), KS11 with IPTG (0.015 mM) (C), and KS11 with IPTG (0.250 mM) (D). Species labeled as bearing PE/PC modifications also carry the hexose-2,4-diacetamido-2,4,6-trideoxyhexose (HexDATDH) dissacharide in its acetylated (ac) form. A complete list of m/z forms and corresponding species of PilE can be found in Table S3 in the supplemental material. Asterisks indicate samples in which pptA was induced with 0.015 mM IPTG (A and C).