Alignment of sulfatase domains from the PptA/LPS PE transferase protein family. (A) Graphical overview of the domain structure of PptA. Color codes: orange, signal peptide (residues 1 to 33) with a hidden transmembrane region (residues 13 to 32); red, transmembrane regions (residues 42 to 61, 68 to 90, 122 to 144, and 156 to 175); green, sulfatase domain PF00884 (residues 230 to 516); blue, Prodom families PD005703 and PD461453; pink, Prodom families PD883089 and PD144483 (found in Lpt6); yellow, Prodom families PD605628 and PD698194 (found in MdoB). The domain structures are similar for all the proteins belonging to Prodom families PD005703 and PD461453. (B) Juxtaposed alignment of the sulfatase domain-containing proteins from Prodom family PD005703. Color codes: red, absolutely conserved residues; green, either S or T; blue, residues conserved only between characterized proteins. Lpt6 is part of the Prodom families PD883089 and PD144483, as indicated by the pink coloring of the residues. MdoB is part of the Prodom families PD605628 and PD698194, as indicated by the yellow coloring of the residues. (C) Alignment of characterized proteins from Prodom family PD461453. The color coding is the same as in panel B. The aligned proteins are as follows: PptA, Q9RMJ3 NEIGO; LptA, Q7DD94 NEIMB; Lpt3, Q9JXJ7 NEIMB; EptB, Q38J80 ECOLI (formerly YhjW); PmrC, YjdB SALTY; CptA, Q7CPC0 SALTY; Lpt6, Q9JWE8 NEIMA; MdoB, P39401 (OPGB) ECOLI.