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. 2007 Oct 19;190(1):387–400. doi: 10.1128/JB.00765-07

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FIG. 9. — PptA activities in structure-based, site-directed PptA mutants. (Top) Immunoblot of whole-cell lysates using polyclonal antibodies specific for PptA. (Middle) Polyclonal antibodies specific for PilE. (Bottom) MAb TEPC-15 specific for PC. Lanes: control, pilV(Fs) (GV1); pptA::kan, pilV(Fs) pptA::kan (KS10); G239A, G239A (KS18); S241A, S241A (KS16); ΔS241, ΔS241 (KS17); H374A, H374A (KS19); G377A, G377A (KS20); S378A, S378A (KS21); H379A, H379A (KS22); S434A, S434A (KS23); D435A, D435A (KS24); H436A, H436A (KS25). As controls for all mutants, immunoblotting was used to ensure that reduced or absent phospho-form modification did not result from decreased PptA stability.