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SDS-PAGE (10% acrylamide) and staining with Coomassie blue of preparations of the hexahistidine-tagged proteins Xon+, SbcB15, and XonA2 obtained by chromatography on Ni-NTA agarose. Lanes 1 and 2, Xon+ (0.2 and 1 μg, respectively); lanes 3 and 4, SbcB15 (0.2 and 1 μg, respectively); lanes 5 and 6, XonA2 (0.2 and 1 μg, respectively); lane M, molecular weight marker proteins with hexahistidine tags. Molecular weights, in thousands, are given on the right.
