FIG. 5.

Agarose gel electrophoresis of 50-bp duplex DNA fragments with 50-nucleotide overhangs of 3′ polarity at each side (200 ng per lane; 6.2 pmol ends) after incubation for 1 min at 0°C followed by 10 s at 37°C with increasing amounts of Xon⁺ (A), XonA2 (B), or SbcB15 (C). Lane 1, no protein; lane 2, 170 ng (3.1 pmol); lane 3, 340 ng (6.2 pmol); lane 4, 680 ng (12.4 pmol); lane 5, 1,020 ng (18.6 pmol); lanes M, duplex DNA marker fragments. The reaction mixtures contained added MgCl₂ (6.7 mM). In panel C, lane 6 contained the same reaction mixture as lane 5 except that the material was treated for 1 min at 60°C with 0.2% SDS before electrophoresis.