TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotypea | Source or reference |
|---|---|---|
| Strains | ||
| S. marcescens | ||
| SM365 | Wild type | Laboratory collection |
| SMH3 | Hem− Strr | 28 |
| SMH3 (hasR) | Hem−hasR::aphA Strr Kmr | 28 |
| SMH3 (hemR) | Hem−hemR::cat Strr Cmr | This study |
| SMH3/pKOBEGA | Hem− λ red functions; Strr Ampr | 28 |
| SMH3 (hasR hemR) | Hem−hasR::aphA hemR::cat Strr Kmr Cmr | This study |
| SMH3 (hasR hemR)/pAM239 | Kmr Strr Spcr | This study |
| SMH3 (hasR hemR)/pAM239-hemR | Kmr Strr Spcr | This study |
| E. coli | ||
| XL1-Blue | F−supE44 hdsR17 recA1 endA1 gyrA46 thi relA1 Δlac F′[ΔproAB lacIqlacZΔM15 Tn10] Tetr | Laboratory collection |
| C600 (ΔhemA) | C600 (F−thr leu lacY thi supE ΔhemA::Km) Kmr | 13 |
| C600 (ΔhemA fur) | C600 (ΔhemA::Km fur::cat) Kmr Cmr | 13 |
| C600 (ΔhemA fur tonB) | C600 (ΔhemA::Km fur::cat tonB trp::Tn10) Kmr Cmr Tetr | 13 |
| C600 (ΔhemA fur exbB) | C600 (ΔhemA::Km fur::cat exbB::Tn10) Kmr Cmr Tetr | This study |
| C600 (ΔhemA)/pAM239 | C600 (ΔhemA::Km)/pAM239 Kmr Spcr | This study |
| C600 (ΔhemA)/pAM238-hasR | C600 (ΔhemA::Km)/pAM238-hasR Kmr Spcr | This study |
| C600 (ΔhemA)/pAM239-hemR | C600 (ΔhemA::Km)/pAM239-hemR Kmr Spcr | This study |
| C600 (ΔhemA fur)/pAM239-hemR | C600 (ΔhemA::Km fur::cat)/pAM239-hemR Kmr Cmr Spcr | This study |
| C600 (ΔhemA fur tonB)/pAM238-hasR/pUC19 | C600 (ΔhemA::Km fur::cat tonB trp::Tn10)/pAM238-hasR/pUC19 Kmr Cmr Tetr Spcr Ampr | This study |
| C600 (ΔhemA fur tonB)/pAM238-hasR/pUCtonBEc | C600 (ΔhemA::Km fur::cat tonB trp::Tn10)/pAM238-hasR/pUCtonBEc Kmr Cmr Tetr Spcr Ampr | This study |
| C600 (ΔhemA fur tonB)/pAM238-hasR/pUChasB6 | C600 (ΔhemA::Km fur::cat tonB trp::Tn10)/pAM238-hasR/pUChasB6 Kmr Cmr Tetr Spcr Ampr | This study |
| C600 (ΔhemA fur tonB)/pAM239-hemR/pUC19 | C600 (ΔhemA::Km fur::cat tonB trp::Tn10)/pAM239-hemR/pUC Kmr Cmr Tetr Spcr Ampr | This study |
| C600 (ΔhemA fur tonB)/pAM239-hemR/pUCtonBEc | C600 (ΔhemA::Km fur::cat tonB trp::Tn10)/pAM239-hemR/pUCtonBEc Kmr Cmr Tetr Spcr Ampr | This study |
| C600 (ΔhemA fur tonB)/pAM239-hemR/pUCtonBSm | C600 (ΔhemA::Km fur::cat tonB trp::Tn10)/pAM239-hemR/pUCtonBSm Kmr Cmr Tetr Spcr Ampr | This study |
| C600 (ΔhemA fur tonB)/pAM239-hemR/pUChasB6 | C600 (ΔhemA::Km fur::cat tonB trp::Tn10)/pAM239-hemR/pUChasB6 Kanr Cmr Tetr Spcr Ampr | This study |
| Plasmids | ||
| pAM239 | Spcr | Laboratory collection |
| pAM238-hasR | pAM238-hasR Spcr | 15 |
| pAM238-hasRΔ45-125 | pAM238-hasRΔ45-125 Spcr | 2 |
| pAM239-hemR | pAM239-hemR Spcr | This study |
| pUC19 | Ampr | Laboratory collection |
| pUCtonBEc | pUC19tonBEc Ampr | 25 |
| pUCtonBSm | pUC19tonBSm Ampr | 25 |
| pUChasB6 | pUC19hasB6 Ampr | 25 |
a
Cmr, chloramphenicol resistance; Strr, streptomycin resistance; Tetr, tetracycline resistance; Ampr, ampicillin resistance; Spcr, spectinomycin resistance; Kmr, kanamycin resistance.