TABLE 1.
φRSA1-specific oligonucleotide primers used for cloning and plasmid construction
| Oligonucleotide primer | Sequence (5′ to 3′)a | Location (nt)b |
|---|---|---|
| attP-Lc | GCAGTATGTGTCCTGGGTGTTTGTCTACCG | 36488-36517 |
| attP-Rc | CCTCTTATCAGAACGCCCCACCTCCC | 37203-37178 |
| ORF33-P1 | GATCCAGCCGGAGAAGTTGGAAGAATCGGG | 25247-25276 |
| ORF36-P2 | GCGAGCCGGTCTCCGTAGTGCATTTTCAAT | 28000-28029 |
| ORF39-P3 | CTCTTCCCCTCACGTTTTTTTCGCGCCTTG | 28997-29026 |
| ORF49-P4 | AGAGCGACAAAGCTTGATTTCCTTTGCTTG | 35718-35689 |
| ORF35-P5 | GTTCAGGCTCACGGAATTCTTCGAAGAACC | 27485-27514 |
a
Sequence underlined is changed to a HindIII site.
b
Numbering corresponds to the sequence with DDBJ accession no. AB276040.
c
A 720-bp region containing attP of φRSA1 was amplified with these primers and used as a probe to confirm the exact integration of φRSA1 in strain MAFF211272 by genomic Southern blot analysis.