FIG. 1.

Glucose represses the gliding motilities of C. perfringens strains isolated from different mammalian hosts. (A) Gliding phenotypes of the three genome-sequenced human pathogen C. perfringens strains 13, SM101, and NCTC8239. Gliding was developed after inoculation of a 5-μl drop of a concentrated middle-log-phase culture of the corresponding strain on BHIA or TYA medium with or without 2% glucose supplementation. Top-bottom photographs were taken after 72 h of anaerobic incubation at 37°C. BHIA, TYA medium without glucose; BHIG and TGY, media supplemented with 2% glucose. Black dotted circles show the diameters of the initial inoculation spots (see Materials and Methods for details). (B) Gliding phenotypes of a collection of human and animal pathogenic C. perfringens strains: 1, NB16; 2, JGS1818; 3, 294442; 4, NCTC 10239; 5, 317206; 6, AHT327; 7, B11; 8, B41; 9, F5603; 10, F4969; and 11, AHT2911 (see Table 1 for the origin and type of infection produced for each pathogenic strain).