TABLE 1.
Strain | Genotype and relevant features | Reference or source |
---|---|---|
10403S | Wild-type strain; serotype 1/2a | 4 |
DP-L1935 | ΔplcB in strain 10403S | 24 |
NF-L943 | PrfA G155S in strain 10403S; the PrfA-dependent genes, including mpl and plcB, are overexpressed | 22 |
HEL-469 | Δmpl in NF-L943 | This study |
HEL-583 | mpl(E350Q) in 10403S | This study |
HEL-587 | mpl(E350Q) in NF-L943 | This study |
HEL-772 | mpl(His6)in NF-L943 | This study |
HEL-793 | HEL-772 ΔplcB | This study |
HEL-798 | mpl(Flag) in NF-L943 | This study |
HEL-800 | mpl(E350Q-Flag) in NF-L943 | This study |
HEL-813 | mpl(E350Q-Flag) integrated at the tRNAArg site in HEL-772 | This study |
HEL-817 | mpl(H437A-His6) integrated at the tRNAArg site in HEL-798 | This study |