Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Nov 2;190(1):363–376. doi: 10.1128/JB.01482-07

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2008, American Society for Microbiology

PMC Copyright notice

FIG. 6. — Accumulation of mutant SpoVE proteins during sporulation. (A) Strains were induced to sporulate in DSM medium and collected 6 h after the onset of sporulation for Western blot analysis using an anti-gfp antibody. wt (wild type), spoVE85 amyE::spoVE-yfp (AH3561); C160R, spoVE85 amyE::spoVE(C160R)-yfp (AH3704); C82R, spoVE85 amyE::spoVE(C82R)-yfp (AH3701); S103N, spoVE85 amyE::spoVE(S103N)-yfp (AH3702); E126G, spoVE85 amyE::spoVE(E126G)-yfp (AH3703); I58N, spoVE85 amyE::spoVE(I59N)-yfp (AH3700); G292R, spoVE85 amyE::spoVE(G292R)-yfp (AH3705); G355D, spoVE85 amyE::spoVE(G355D)-yfp (AH3706); no YFP, wild-type spoVE (MB24). (B) Strains were sporulated by resuspension and collected at h 3 of sporulation for Western blot analysis using an anti-gfp antibody. wt (wild type), ΔspoVE::tet amyE::P_spoVE-spoVE-gfp (JDB1835); G335A, ΔspoVE::tet amyE::P_spoVE-spoVE(G335A)-gfp (JDB1845); S341A, ΔspoVE::tet amyE::P_spoVE-spoVE(S341A)-gfp (JDB1846); G343A, ΔspoVE::tet amyE::P_spoVE-spoVE(G343A)-gfp (JDB1847); N322A, ΔspoVE::tet amyE::P_spoVE-spoVE(N322A)-gfp (JDB1848); E271A, ΔspoVE::tet amyE::P_spoVE-spoVE(E271A)-gfp (JDB1849); W69A, ΔspoVE::tet amyE::P_spoVE-spoVE(W69A)-gfp (JDB1850); K76A, ΔspoVE::tet amyE::P_spoVE-spoVE(K76A)-gfp (JDB1851); T173A, ΔspoVE::tet amyE::P_spoVE-spoVE(T173A)-gfp (JDB1852). Molecular mass markers, in kDa, are on the left of the panels.