TABLE 2.

Phenotypes of SpoVE mutants^a

Genotype	CFU/ml preheat	CFU/ml postheat	% Sporulation	Protein accumulationb	Localization ratioc	Localizationd
Wild type (PY79)	1.9 × 108	1.4 × 108	74	N/A	N/A	N/A
ΔspoVE::tet	8.8 × 107	0	0	N/A	N/A	N/A
spoVE-gfp	2.1 × 108	1.4 × 108	67	++++	3.51 ± 0.20	OFM
spoVE(G335A)-gfp	1.3 × 108	0	0	++++	3.34 ± 0.21	OFM
spoVE(S341A)-gfp	1.6 × 108	0	0	+++++	3.32 ± 0.33	OFM
spoVE(G343A)-gfp	1.2 × 108	0	0	+++++	3.28 ± 0.19	OFM
spoVE(N322A)-gfp	9.9 × 107	1.6 × 105	0.16	+++++	3.20 ± 0.12	OFM
spoVE(E271A)-gfp	1.2 × 108	0	0	+++++	3.11 ± 0.22	OFM
spoVE(I58N)-yfp	2.6 × 108	0	0	++	ND	OFM
spoVE(C82R)-yfp	3.0 × 108	0	0	ND	ND	ND
spoVE(S103N)-yfp	2.5 × 108	0	0	+++++	ND	OFM
spoVE(E116G)-yfp	1.4 × 108	0	0	+++++	ND	OFM
spoVE(C160R)-yfp	9.2 × 107	0	0	+	ND	ND
spoVE(G292R)-yfp	1.3 × 108	0	0	++	ND	MCM
spoVE(G355D)-yfp	1.6 × 108	0	0	+	ND	MCM
spoVE(W69A)-gfp	1.0 × 108	6.0 × 101	0.00006	++	1.97 ± 0.15	MCM
spoVE(K76A)-gfp	7.3 × 107	3.8 × 102	0.0005	++	1.94 ± 0.13	MCM
spoVE(T173A)-gfp	9.7 × 107	1.8 × 102	0.0002	++	1.89 ± 0.28	MCM

^aData represent the results of one typical heat kill experiment. Each strain was grown in DSM for 24 h at 37°C and then exposed to 80°C for 20 min.

^bSpoVE-GFP or SpoVE-YFP accumulation at h 6 of sporulation. ND, not detectable.

^cRatio between all four mother cell membranes (including forespore) and the two sporangial cell membranes. See text and Fig. 5 for details.

^dSpoVE localization as assessed by analysis with GFP and YFP fusions. See Fig. 5. N/A, not assessed; OFM, outer forespore membrane; MCM, mother cell membranes.