Figure 3.

The determination of tubulin-critical concentrations. Six assembly groups were analyzed: (i) tubulin alone (open circles); (ii) tubulin plus TMAO (close circles); (iii) tubulin plus tau (open triangles); (iv) tubulin, tau, plus TMAO (closed triangles); (v) tubulin plus phosphorylated tau (open squares); (vi) tubulin, phosphorylated tau, plus TMAO (closed squares). The detailed assembly conditions are described in Materials and Methods. The lines are the results after a linear curve fitting processing for each group.