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. 2007 Nov 7;15(1):147–153. doi: 10.1128/CVI.00363-07

FIG. 1.

FIG. 1.

Recombinant proteins B1t and B2t. (a) The coding regions of these proteins were obtained by RT-PCR of E. granulosus cDNA. Primers were based on the nucleotide sequences from antigens B1 and B2 available in GenBank (accession no. AF143813 and U15001, respectively) to amplify the sequence encoding the C-terminal portion of antigens B1 and B2 (white boxes) but not the coding region for the putative signal peptides from both sequences (black boxes). Numbers indicate amino acid positions. (b) The resulting RT-PCR products encoding the proteins B1t and B2t were subcloned in the pGEX-4T1 vector and expressed in E. coli BL21 CodonPlus RIL cells. (c) The purified proteins after thrombin cleavage are shown in a 15% acrylamide Coomassie blue-stained gel. Lane 1, molecular mass markers; lane 2, B1t protein; and lane 3, B2t protein. Relative molecular masses are shown to the left of the gel.