FIG. 6.

Inhibitory effect of resveratrol on impaired phagocytosis of bacteria mediated by SRs and CLRs. (A) THP-1 cells incubated for 24 h with 100 nM FSL-1 were treated with 100 μM resveratrol for 1 h in a serum-free condition. The expression of mRNAs for MSR1, CD36, DC-SIGN, Dectin-1, and β-actin was confirmed by RT-PCR using total RNA isolated from the cells. Representative data are from more than five independent experiments. (B) 293/DC-SIGN cells were incubated for 0, 30, 60, 90, or 120 min with 100 μM resveratrol. The cells were then lysed in SDS sample buffer, and proteins were separated by electrophoresis on 10% SDS-polyacrylamide gels and transferred to a nitrocellulose membrane. The membrane was reacted with anti-DC-SIGN mAb or anti-β-actin mAb. (C) 293/DC-SIGN cells were treated with the indicated doses of resveratrol for 1 h and given heat-killed FITC-conjugated E. coli or S. aureus at various cell:bacterium ratios. The mean values and SD from triplicate experiments are shown. The statistically significant difference from vehicle was assessed by Student's t test: *, P < 0.05; **, P < 0.01. Representative data are from more than three independent experiments. Veh, vehicle.