TABLE 1.
Assay | Sp acta (nmol/min/mg of protein) (mean ± SD) |
---|---|
SDH | 103 ± 16 |
Succinate-quinone reductase | |
dUQ (anaerobic) | 98.9 ± 12 |
dRQ (anaerobic) | 16.6 ± 3.5 |
Quinol-fumarate reductase (decyl rhodoquinol) (anaerobic) | 60.2 ± 18 |
NADH oxidase | 9.1 ± 2.1 |
NADH oxidase with: | |
2 mM KCN | 7.3 ± 1.5 |
100 mM malonate | 4.4 ± 0.4 |
2 mM KCN and 100 mM malonate | 1.7 ± 0.7 |
Ubiquinol-1 oxidase | 4.4 ± 0.6 |
TMPD oxidase | 12.6 ± 6.3 |
NADH-fumarate reductase (anaerobic) | 45.0 ± 8.1 |
NADH-quinone reductase | |
dUQ (anaerobic) | 32.1 ± 2.7 |
dRQ (anaerobic) | 61.3 ± 4.3 |
Specific activities were obtained from at least three independently isolated mitochondria.