FIG. 3.

Structure of the DNA cassettes used to integrate the Tet-inducible SAP genes into the ADH1 locus of C. albicans strain SAP2MS4B (in this example shown for SAP2 expression). Bent arrows symbolize promoters (P); filled circles represent T_ACT1, which serves for proper transcription termination of the Candida-adapted, reverse tetracycline-dependent transactivator (cartTA) and the target genes in this cassette (37). Only the following relevant restriction sites used to construct the plasmids or to excise the whole cassette from the vector backbone are shown: A, ApaI; B, BamHI; Bg, BglII; S, SalI; ScII, SacII.