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. 2007 Oct 22;52(1):146–156. doi: 10.1128/AAC.01072-07

FIG. 4.

FIG. 4.

Tet-inducible expression of SAP1, SAP2, SAP3, SAP4, SAP5, SAP6, SAP8, and SAP9ΔC493 allows growth of a sap2Δ mutant in YCB-BSA. Overnight cultures of strains SAP1ex4A, SAP2ex4A, SAP3ex4A, SAP4ex4A, SAP5ex4A, SAP6ex4A, SAP8ex4A, and SAP9ΔC2ex4A were diluted 1:100 in YCB-BSA without (−Dox) or with (+Dox) doxycycline. In addition, the strains were also incubated in the presence of both doxycycline and pepstatin (+Dox/Pep). Untransformed sap2Δ mutant SAP2MS4B served as a control. The tubes were photographed, and the optical densities at 600 nm of the cultures were measured after 48 h (for SAP2MS4B and strains expressing SAP1, SAP2, SAP3, and SAP8) or 96 h (for strains expressing SAP4, SAP5, SAP6, and SAP9ΔC493). The OD600 values are given below each tube. The two independently constructed transformants for Tet-inducible expression of the individual SAP genes behaved identically, and only one of them is shown in each case.