FIG. 5.

Detection of individual Saps in the culture supernatants of SAP-expressing transformants. sap2Δ mutant SAP2MS4B and strains expressing specific SAP genes, as indicated by the numbers above the lanes, were cultivated in YCB-BSA medium with doxycycline (for details, see Materials and Methods). (A) Culture supernatants of the strains were analyzed on an SDS-polyacrylamide gel stained with colloidal Coomassie dye. Lane M, molecular size markers. (B and C) Detection of the Saps in the supernatants of the analyzed strains by Western immunoblotting with antibodies raised against Sap2p (B) and Sap5p (C).