TABLE 2.
Primers used in this study
| Primer | Sequencea |
|---|---|
| SAP2M | 5′-CTGCCTTGACCGCGGATGAAGGGGTG-3′ |
| SAP2N | 5′-GGGCCCATGACGAGCTCTAGTTTGAGC-3′ |
| SAP2P1 | 5′-TTGTTGGGCCCGTTGTCAATTTATGGGCCGATCTG-3′ |
| SAP2P5 | 5′-ATATAGGGCCCGCATTTGAATAAACGGCATC-3′ |
| SAP2E | 5′-CCACCCCTTCATCTGCAGTCAAGGCAGAAATAC-3′ |
| SAP1ex1 | 5′-TCTAGTCGACAATGTTTTTAAAGAATATTTTCAT-3′ |
| SAP1ex2 | 5′-AGTAGGGATCCCTAGGTAAGAGCAGCAATGTTTGAAGC-3′ |
| SAP2ex1 | 5′-ACCAGTCGACAATGTTTTTAAAGAATATTTTCAT-3′ |
| SAP2ex2 | 5′-ACCCCGGATCCTTAGGTCAAGGCAGAAATACTGGAAGC-3′ |
| SAP3ex1 | 5′-ATATGTCGACAATGTTTTTAAAAAATATCTTTAT-3′ |
| SAP3ex2 | 5′-TGTACGGATCCCTAAGTAAGAGCAGCAATGTTAGAAGC-3′ |
| SAP4ex1 | 5′-CTCACTCGAGAATGTTCTTACAAAATATCTTGAG-3′ |
| SAP4ex2 | 5′-ACCAAGGATCCCTAATTAATAGCAACAATGTTAGACTG-3′ |
| SAP56ex1 | 5′-CTCAGTCGACAATGTTCTTGAAAAATATCTTGAG-3′ |
| SAP5ex2 | 5′-GTCAAGGATCCTTAATTAATAGCAACAATGTCAGACTCGG-3′ |
| SAP6ex2 | 5′-ACCAAGGATCCCTAATTAATAGCACCAATGTTAGACTC-3′ |
| SAP7ex1 | 5′-GAAGGTCGACAATGCAAAGAGTATTAGAGTTATT-3′ |
| SAP7ex2 | 5′-TTCTAGGATCCTTACTCGATAGGAACAACGGCATGGTT-3′ |
| SAP8ex1 | 5′-AACCGTCGACAATGGTCTCCATTATTACTTTTAC-3′ |
| SAP8ex2 | 5′-GAACAGGATCCCTATAAAGTAGAAATACTTGAAGAAGT-3′ |
| SAP9ex1 | 5′-TTTCGTCGACAATGAGACTCAATTCTGTTGCGTT-3′ |
| SAP9ex2 | 5′-TCTCTGGATCCTTAAACCAAAACATAGTAGGATATCAA-3′ |
| SAP9ex3 | 5′-TATAGGATCCTTATGAATGACGTGTGCTGGTAC-3′ |
| SAP9ex4 | 5′-TATAGGATCCTTAACCAATGACTTCAATCGATTCC-3′ |
| SAP10ex1 | 5′-TATAGTCGACAATGGACCTAGTAATAATGAATTT-3′ |
| SAP10ex2 | 5′-ATTCTGGATCCCTATATAATATGTATATACACGAGGAA-3′ |
| SAP10ex3 | 5′-TATAGGATCCTTAGCTAGTAGTGTTCTTGTCCATGTTC-3′ |
| SAP10ex4 | 5′-TATAGGATCCTTAGTTTAAAATCTCTTCAATATCTTCGTCTTC-3′ |
| SAP5CF | 5′-CGGGGATCCATGGTTAACTGTCGGTTTAAG-3′ |
| SAP5CR | 5′-CGCTCGAGATTAATAGCAACAATGT-3′ |
a
Restriction sites introduced into the primers are underlined; the start and stop (reverse sequence) codons of the SAP genes are in bold.