TABLE 3.
PCR results with various tet(32), tet(O), and tet(W) primers
| PCR test | Forward primer | Reverse primer | Annealing Tm (°C) | Product length (bp) | Presence/absence of strain(s)
|
|||
|---|---|---|---|---|---|---|---|---|
| B. thermophilum | L. johnsonii | tet(W) | tet(O) | |||||
| 1 | tetO −372F | tetO 161R | 58 | 533 | + | + | − | + |
| 2 | tetO −204F | 58 | 365 | + | + | − | + | |
| 3 | tetO −372F | tetW 589R | 58 | 961 | + | + | − | − |
| 4 | tetO −204F | 58 | 793 | + | + | − | − | |
| 5 | tetO 144F | 58 | 445 | + | + | − | − | |
| 6 | tetO −372F | tetW 1757R | 58 | 2,129 | + | − | − | − |
| 7 | tetO −204F | 58 | 1,961 | + | − | − | − | |
| 8 | tetW-Fw | 60 | 1,695 | − | − | + | − | |
| 9 | tetO 144F | 58 | 1,613 | + | − | − | − | |
| 10 | tetW 384F | 60 | 1,373 | + | − | + | − | |
| 11 | tetW 1278F | 60 | 479 | + | − | + | − | |
| 12 | tetO −204F | tetW 1890R | 58 | 2,094 | + | − | − | − |
| 13 | tetO 14F | 60 | 1,876 | + | − | − | − | |
| 14 | tetO 144F | 58 | 1,746 | + | − | − | − | |
| 15 | tetW 384F | 60 | 1,506 | + | − | + | − | |
| 16 | tetW 1278F | 60 | 612 | + | − | + | − | |
| 17 | tetO −204F | tetO 1917R | 58 | 2,121 | − | + | − | + |
| 18 | tetO 14F | 60 | 1,903 | − | + | − | + | |
| 19 | tetW-Fw | 60 | 1,855 | + | − | − | − | |
| 20 | tetO 144F | 58 | 1,773 | − | + | − | + | |
| 21 | tetW 384F | 60 | 1,533 | + | + | − | − | |
| 22 | tet32 1107F | 58 | 810 | + | + | − | − | |
| 23 | tetW 1278F | 60 | 612 | − | − | − | − | |