TABLE 2.

Effect of MoistureLoc or MultiPlus solution on metabolic activities of biofilms formed by two species of Fusarium or C. albicans on lotrafilcon A lens^a

Strain	Lens care solution	Activity for incubation time (h) of:				P valueb
		4		20
		Mean ± SD	% Growth	Mean ± SD	% Growth
FSSC 1-b MRL8609	None	0.0403 ± 0.0015	100.0	0.0377 ± 0.0006	100.0	0.047
	MoistureLoc	0.0190 ± 0.0010	47.1	0.0140 ± 0.0010	37.1	0.004
	MultiPlus	0.0197 ± 0.0012	48.8	0.0093 ± 0.0006	24.7	0.0001
FOSC 3-a MRL8996	None	0.0433 ± 0.0025	100.0	0.0483 ± 0.0029	100.0	0.087
	MoistureLoc	0.0230 ± 0.0046	53.1	0.0193 ± 0.0031	39.9	0.313
	MultiPlus	0.0223 ± 0.0006	51.5	0.0263 ± 0.0023	54.4	0.044
C. albicans SC5314	None	0.1483 ± 0.0124	100.0	0.1310 ± 0.0096	100.0	0.129
	MoistureLoc	0.1787 ± 0.0119	120.4	0.1337 ± 0.0159	102.1	0.017
	MultiPlus	0.1797 ± 0.0040	121.1	0.1777 ± 0.0112	135.6	0.785

^aBiofilms were formed using an FSSC 1-b (MRL8609), FOSC 3-a (MRL8996), or C. albicans SC5314 isolate on lotrafilcon A lenses as described in Materials and Methods. The effect of MoistureLoc or MultiPlus lens care solution on the metabolic activities of fungal biofilms was determined using the XTT-based metabolic activity assay. Percent growth and metabolic activity (measured as optical density at 492 nm) were calculated for each lens care solution with respect to metabolic activity of biofilm grown in the absence of the disinfectant (which was considered to be 100% activity). Data represent means ± SDs for three separate experiments.

^bP values were obtained for comparison of metabolic activities of biofilms formed by each isolate with incubation with lens care solution for 4 h or 20 h.