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. 2007 Oct 29;52(1):157–163. doi: 10.1128/AAC.00904-07

FIG. 3.

FIG. 3.

ATP-mediated excision of AZT-MP from an RNA/DNA T/P by WT and mutant HIV-1 RTs. (A) Isotherm for ATP-mediated excision of AZT-MP by WT (○), M184V (▿), AZTr (□), AZTr/M184V (⋄), and AZTr/M184V/G333D (▵) HIV-1 RTs. Excision products were analyzed at 0, 2, 5, 8, 12.5, 20, 30, and 45 min, respectively. Data are averages of three separate experiments. (B) Rate of degradation of RNA template by RNase H activity of WT and mutant HIV-1 RTs. Products were analyzed at 0, 2, 5, 8, 12.5, 20, 30, and 45 min, respectively. Rates were determined by scanning the uncleaved RNA substrate (panel C) as a function of time. (C) Representative autoradiograms of RNase H activities of WT and mutant HIV-1 RTs. Assays were carried out as described in Materials and Methods. The time points in this experiment were 0, 5, 8, 12.5, 20, 30, and 45 min, respectively. nt, nucleotides.