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. 2007 Oct 29;52(1):157–163. doi: 10.1128/AAC.00904-07

FIG. 4.

FIG. 4.

Steady-state ATP-mediated excision of AZT-MP from a DNA/DNA T/P by WT and mutant HIV-1 RTs. (A) Isotherm for ATP-mediated excision of AZT-MP by WT (○), M184V (▿), AZTr (□), AZTr/M184V (⋄), and AZTr/M184V/G333D (▵) HIV-1 RTs. (B) Isotherm for ATP-mediated excision of AZT-MP by WT (○), AZTr/M184V (⋄), AZTr/K65R (▾), AZTr/M184V/G333D (▵), and AZTr/K65R/G333D (•) HIV-1 RTs. For both isotherms, excision products were analyzed at 0, 15, 30, 45, 60, 90, 120, 150, and 180 min, respectively. Data are averages of three separate experiments. (C) Representative autoradiographs indicating the relative abilities of WT and mutant HIV-1 RTs to excise AZT-MP and rescue DNA synthesis under steady-state conditions. The time points in this experiment were 15, 30, 45, 60, 120, 150, and 180 min, respectively.