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. 2007 Nov 9;7(1):38–48. doi: 10.1128/EC.00207-07

FIG. 8.

FIG. 8.

Overexpression of flbB inhibits conidiation. (A) Photographs of the colonies of control (TNI22.1) and alcA(p)::flbB (RNI24.5) strains grown on MMA plus glucose (MMG; noninducing) and MMA plus yeast extract (MMT+5Y; threonine-inducing alcA promoter, with 5 g/liter yeast extract enhancing growth) for 5 days are shown. Overexpression of flbB resulted in a blockage in conidiation. (B) Control (TNI22.1) and alcA(p)::flbB (RNI24.5) strains were grown in liquid glucose medium at 37°C and 250 rpm for 18 h and then transferred onto solid medium with threonine (MMT; inducing) as the sole carbon source. Samples were collected at 18 h of vegetative growth (Veg) and 6, 12, and 24 h after asexual developmental induction (Asex) on MMT. Total RNA was isolated and subjected to Northern blot analyses for the levels of brlA and flbB mRNAs. Equal loading of total RNA was evaluated by ethidium bromide staining of rRNA. (C) Close-up views of control (TNI22.1) and alcA(p)::flbB (RNI24.5) strains grown on MMT for 48 h after transfer onto solid MMT. The control strain produced conidiophores abundantly (arrows), whereas alcA(p)::flbB strain (RNI24.5) formed elongated aerial hyphae without conidiophores.