FIG. 3.

Disruption of the glucose gradient perturbs mat formation. The glucose gradient set up within the growing mat was perturbed by adding 50 μl of 40% glucose or water as a negative control to a pit dug in the agar at day 3 (3d). After two more days (5d) the plates were examined and photographed. The black circles mark the pit in the agar where the glucose or water was added.