FIG. 6.

Mutants in glucose-sensing pathways have variable effects on FLO11 expression. Wild-type and mutant strains were grown in YPD medium to logarithmic phase (OD₆₀₀ of 0.5 to 1.0) (A) or post-logarithmic phase (OD₆₀₀ of ∼4.0) (B) where the cells are beginning to experience the diauxic shift. The cells were then collected by centrifugation, and RNA was extracted, Northern blotted, and probed for FLO11. The results were visualized and quantified on a Storm Phosphorimager with ImageQuant software. The quantification of FLO11 was normalized to the amount of rRNA isolated from each sample. The graphs represent the results of three biological replicates and are presented as the percentage of FLO11 expressed by the wild-type strain (WT) at log phase. The flo11Δ and sfl1Δ (overexpresses FLO11) strains were included as controls for specificity of the FLO11 probe.