FLO11 is expressed in the rim, hub, and intermediate (middle) regions of the mat, but the other FLO genes are not expressed. (A) Real-time RT-PCR was used to measure the expression of FLO11 and the other FLO genes, and the levels of expression were normalized to ACT1. (B) Real-time RT-PCR was used to measure the expression of FLO11, the other FLO genes, and ACT1, and the levels of expression were normalized to SNR190.