Fig. 5.

RF-C11 interacts with N-recognins. (A) ^hUBR1¹⁻⁴⁵³ was immobilized onto Ni⁺⁺-coated microplates and incubated with RF-C11b or GV-C11b, followed by monitoring streptavidin-IR signals. (B) RF-C11b or GV-C11b were immobilized onto streptavidin-coated microplates and incubated with ^hfUBR1¹⁻⁴⁵³, and anti-FLAG antibody-conjugated IR signals were measured. (C) ^hUBR1¹⁻⁴⁵³ and ^hfUBR1¹⁻⁴⁵³ were expressed in wheat germ extracts and subjected to anti-His-6 and FLAG immunoblotting. (D) RF-C11b can pull down endogenous N-recognins. RF-C11b or GV-C11b, conjugated to NeutrAvidin-coated microbeads, was incubated with rat testes extracts in the presence or absence of 2 mM dipeptides, followed by precipitation, washing, separation, and immunoblotting for UBR1 and UBR5.