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Frequently methylated CpG islands in lung SCCs. Verification of CpG island DNA methylation markers in normal lung tissue and matching stage I SCC samples was conducted by bisulfite-based COBRA assays. Methylation differences between SCCs (T) and matching normal tissues (N) were analyzed by COBRA assays of the indicated gene targets. −, control digestion with no BstUI; +, BstUI-digested samples. Digestion by BstUI indicates methylation of the sequence that was tested.
