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. 2008 Jan 2;105(1):258–263. doi: 10.1073/pnas.0710779105

Fig. 2.

Fig. 2.

Binding affinity is a function of pH and dsRNA size. (A and B) TLR3ecd binding to immobilized dsRNA as determined by SPR. (A) Binding of TLR3ecd (40 μg/ml) to dsRNA (540 bp) increases with acidity. Arrows indicate the points at which TLR3ecd injection began and ended with the change back to buffer alone. (B) Affinity of TLR3ecd (40 μg/ml) binding at pH 6.0 increases with the length of the immobilized dsRNA. (C) The amount of TLR3ecd bound to dsRNA at equilibrium was determined by SPR, at several concentrations of soluble (free) TLR3ecd, and data were plotted for Scatchard analysis. Downward curvature suggests positive cooperativity.