Table 1.

Affinity of TLR3ecd binding to dsRNA

dsRNA length, bp	Kd_apparent, nM
	pH 6.5	pH 6.0	pH 5.5
39	2,250 ± 70	510 ± 23	72.6 ± 1.7
48	2,610 ± 80	156 ± 7	13.6 ± 0.3
139	487 ± 12	31.7 ± 0.6	9.19 ± 0.21
540	782 ± 15	28.2 ± 0.9	4.64 ± 0.11

Apparent equilibrium dissociation constants (K_{d_apparent}) were calculated for each dsRNA size and pH by fitting kinetic data from SPR experiments to a simple single-step binding model (A+B ↔ AB). See SI Table 2 for a similar analysis using a cooperative binding model. The standard error associated with each calculation is indicated.