Fig. 3.

Disruption of Rv2224c prolongs survival and reduces lung immunopathology of infected mice. (A and B) Single infections of C57BL/6 mice with wild type or Rv2224c::tn and Mtb cfus in lungs (A) and spleens (B) over time. Four mice were used per strain for each time point. cfu data are represented as fold increase in cfus relative to cfus on day 1. (C) Survival of C57BL/6 mice infected with wild type or Rv2224c::tn. Twelve mice were infected in each group. Median survival time was 195 days (wild type) and 336 days (Rv2224c::tn) (P < 0.0001 by log-rank test). (D) (Top) Gross pathology of lungs at 20 weeks after infection. (Middle and Bottom) H&E-stained lung tissue sections from wild-type- or mutant-infected mice at 20 weeks. (Scale bars, Middle, 1 mm; Bottom, 50 μm.)