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. 1999 Aug 17;96(17):9527–9532. doi: 10.1073/pnas.96.17.9527

Figure 1.

Figure 1

Sequence comparisons of the accessory subunit of Drosophila polγ with mammalian homologs and with class IIa aaRSs. (A) Sequence alignment between the C-terminal regions of the accessory subunit of D. melanogaster polγ (Dm) (7) and its homologs from Homo sapiens (Hs) (7) and Mus musculus (Mm, GenBank accession no. AF006072). Residues are shaded based on degree of similarity, with dark shading indicating identical residues and light shading indicating conservative substitutions within the categories of the simplification matrix from the UW-GCG package (12): negatively-charged residues and derivatives (Asp, Asn, Glu, Gln); positively charged residues (His, Lys, Arg); small hydrophobic residues (Leu, Ile, Val, Met); large hydrophobic residues (Phe, Tyr, Trp); Cys; and other residues (Pro, Ala, Gly, Ser, Thr). Open boxes indicate loosely conserved residues, i.e., those conserved as hydrophobic or hydrophilic. ⋄ marks every 10th residue in the Drosophila polγ-β sequence. (B) Comparison of the Drosophila polγ-β sequence with the T. thermophilus (Tt) prolyl-RS (13), glycyl-RS (PDB ID 1ati) (24), and histidyl-RS sequences (PDB ID 1adj) (18). Sequences are shaded as in A, with underlined residues in polγ indicating conservation of hydrophobic/hydrophilic character between the accessory subunit and the RSs.