Fig. 4.
An exogenous acid load reveals a defect in pHi regulation in Slc4a10 KO slices with a parallel in network activity. (A) Grand averages of pHi responses upon an intracellular acid load induced by bath application of 20 mM propionate for 13 min (horizontal bar) from CA3 stratum pyramidale (12 Slc4a10 KO slices, gray line; 10 WT slices, black line). Upward deflections indicate an increase in pHi. (B–D) Mean ± SEM values of the peak acid shift in pHi induced by propionate (B), recovery of pHi in percentage of peak acidosis by the end of the propionate application (C), and amplitude of the alkaline overshoot during propionate washout (D). *, P < 0.05; **, P < 0.01. (E) Effect of 10 mM propionate on the occurrence of 4-AP-induced interictal-like events in WT (filled circles; n = 20 slices) and Slc4a10 KO slices (open circles; n = 27 slices) shown as a relative mean (+ SEM) frequency calculated from a sliding 3-min time window. (Insets) Sample traces of interictal-like events recorded with field potential electrodes in the hippocampal CA3 pyramidal cell layer under control conditions (Left) and during 25-min propionate application (Right) in the WT (Upper) and Slc4a10-KO (Lower) slices.