Fig. 5.

Expression of the Aytl members in erythroid cells. Reverse transcription of Aytl1, Aytl2, and Aytl3 from total RNA of isolated mouse reticulocytes (lane R) and Friend cells (lane FC) (see Materials and Methods). RT-PCR products were separated on agarose gel and stained with ethidium bromide. In each gel, the 1-kbp DNA molecular weight (MW) standard (Invitrogen) is shown. Note that the two faint bands smaller than the major products of Aytl1 (lane FC) correspond to alternatively spliced variants 2 and 3, shown in SI Fig. 9.