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Analytical sensitivity of the Tso31 nested PCR. Lanes 1 and 10, 100-bp ladders. DNA extracted from T. solium cysts was spectrophotometrically quantitated and diluted. PCR was carried out using different DNA concentrations as follows; lanes 2 to 9, 10 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, 10 fg, and 1 fg, respectively.
