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Purified recombinant N protein expressed in E. coli. (A) Results of SDS-PAGE (5 to 20% gradient gel) for recombinant N protein. (B) Results of Western blot analysis for recombinant N protein that reacted with anti-SARS CoV nucleocapsid MAb (catalog no. 654; IMGENEX Co. Ltd.). Lane 1, molecular mass marker; lane 2, purified recombinant His6-tagged N protein.
