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. 2007 Nov 21;46(1):302–310. doi: 10.1128/JCM.01006-07

FIG. 4.

FIG. 4.

Evaluation of sandwich ELISA system with various MAbs. (A) Evaluation of sandwich ELISA by color detection system using 25 different combinations of anti-SARS CoV N protein MAbs. Twenty nanograms of recombinant full-length N protein was measured by different combination of MAbs with sandwich ELISA systems. Asterisks indicate the best five results among the 25 ELISAs with different combinations of MAbs. *1, ELISA with I-23/II-12 (capture MAb/detection MAb); *2, ELISA with II-21/II-3; *3, ELISA with II-12/I-23; *4, ELISA with II-21/I-23; and *5, ELISA with I-16/I-23. (B) Standard antigen ELISA curves determined with purified recombinant N protein by sandwich ELISA (CLEIA) using five kinds of MAb pairs (capture MAb/detecting MAb): •, I-23/II-12; ▪, II-21/I-23; ▴, II-12/I-23; ⧫, II-21/II-3; and ○, I-16/I-23. Serial dilutions of recombinant N protein were measured in triplicate by CLEIA. Results show the means of triplicate results. Error bars indicate the range of standard deviations.