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. 2007 Nov 21;46(1):302–310. doi: 10.1128/JCM.01006-07

FIG. 5.

FIG. 5.

Sensitivity of CLEIA in detecting SARS CoV N antigen. (A) Limiting dilution experiment with cultured SARS CoV (HKU-39849) for detecting SARS CoV N antigen by CLEIA using I-23 as capture MAb and II-12 as detecting MAb. Twofold dilutions of cell culture lysates of SARS CoV were measured in triplicate by the CLEIA. Results show mean values of the triplicates. Error bars express the range of 2× standard deviation values. The limit of detection of CLEIA is 0.087 TCID50/ml (a coarse dotted line), which is the minimum concentration (TCID50/ml) of cultured SARS CoV (HKU-39849) giving the signal value of the mean minus 2.0 standard deviations, which is higher than that of the mean plus 2.0 standard deviations of 0.0 TCID50/ml (a fine dotted line). (B) Limiting dilution experiment with cell culture lysates of common human CoV (229E [▪] or OC43 [▴]). Serial dilutions of recombinant N protein were measured in triplicate by the CLEIA. Results show mean values of the triplicates. Error bars indicate 2× standard deviation values.