TABLE 2.
Primers for synthesizing cDNA of SARS CoV N protein
| Primera | Sequence |
|---|---|
| S1 | 5′-ATGAGCGATAACGGCCCGCAGAGCAACCAGCGTAGCGCGCCGCGTATTACCTTTGGCGGCCCGACCGATAGCACCGATAACAACCAGAACGGCGGCCG-3′ |
| S2 | 5′-GTTTACCGCGCTGACCCAGCATGGCAAAGAAGAACTGCGTTTTCCGCGTGGCCAGGGCGTGCCGATTAACACCAACAGCGGCCCGGATGATCAGATTGGC-3′ |
| S3 | 5′-CGCGTTGGTATTTTTATTATCTGGGCACCGGCCCGGAAGCGAGCCTGCCGTATGGCGCGAACAAAGAAGGCATTGTGTGGGTGGCGACCGAAGGCGCGCTG-3′ |
| S4 | 5′-CAGCTGCCGCAGGGCACCACCCTGCCGAAAGGCTTTTATGCGGAAGGCAGCCGTGGCGGCAGCCAGGCGAGCAGCCGTAGCAGCAGCCGTAGCCGTGGC-3′ |
| A1 | 5′-GCTGGGTCAGCGCGGTAAACCAGCTCGCGGTGTTGTTCGGCAGGCCCTGCGGACGACGCTGTTTCGGACGCGCGCCGTTACGGCCGCCGTTCTGGTTGTTATC-3′ |
| A2 | 5′-GATAATAAAAATACCAACGCGGGCTCAGTTCTTTCATTTTGCCATCGCCGCCACGCACACGACGGGTCGCACGACGATAATAGCCAATCTGATCATCCGGGC-3′ |
| A3 | 5′-GTGGTGCCCTGCGGCAGCTGCAGCACGGTCGCCGCGTTGTTGTTCGGGTTACGGGTGCCAATATGATCTTTCGGGGTGTTCAGCGCGCCTTCGGTCGCCAC-3′ |
| A4 | 5′-CAGCGCGGTTTCGCCGCCGCCGCTCGCCATACGCGCCGGGCTGTTGCCACGGCTGCTGCCCGGGGTGCTGTTACGGCTGTTGCCACGGCTACGGCTGCTGC-3′ |
| S5 | 5′-ACAGCCGTAACAGCACCCCGGGCAGCAGCCGTGGCAACAGCCCGGCGCGTATGGCGAGCGGCGGCGGCGAAACCGCGCTGGCGCTGCTGCTGCTGGATCGTCTG-3′ |
| S6 | 5′-AAAGCGCGGCGGAAGCGAGCAAAAAACCGCGTCAGAAACGTACCGCGACCAAACAGTATAACGTGACCCAGGCGTTTGGCCGTCGTGGCCCGGAACAGACCCAGGG-3′ |
| S7 | 5′-GCGCAGTTTGCGCCGAGCGCGAGCGCGTTTTTTGGCATGAGCCGTATTGGCATGGAAGTGACCCCGAGCGGCACCTGGCTGACCTATCATGGCGCGATTAAAC-3′ |
| S8 | 5′-ACCTTTCCGCCGACCGAACCGAAAAAAGATAAAAAAAAAAAAACCGATGAAGCGCAGCCGCTGCCGCAGCGTCAGAAAAAACAGCCGACCGTGACCCTGC-3′ |
| A5 | 5′-GCTCGCTTCCGCCGCGCTTTTTTTGGTCACGGTCTGGCCCTGCTGCTGCTGGCCTTTGCCGCTCACTTTGCTTTCCAGCTGGTTCAGACGATCTAGCAGCAGCA-3′ |
| A6 | 5′-CGCGCTCGGCGCAAACTGCGCAATCTGCGGCCAATGTTTATAATCGGTGCCCTGACGAATCAGATCCTGATCGCCAAAGTTGCCCTGGGTCTGTTCCGGGCC-3′ |
| A7 | 5′-GGTTCGGTCGGCGGAAAGGTTTTATACGCATCAATATGTTTGTTCAGCAGAATCACGTTATCTTTAAACTGCGGATCTTTATCATCCAGTTTAATCGCGCCATGATAG-3′ |
| A8 | 5′-TTACGCCTGGGTGCTATCCGCGCTCGCGCCGCTCATGCTGTTCTGCAGCTGACGGCTAAAATCATCCATATCCGCCGCCGGCAGCAGGGTCACGGTCGGCTG-3′ |
| NS | 5′-CCCGGATCCATGAGCGATAACGGCCCGCA-3′ |
| NA | 5′-CCCACAGCCGTAACAGCACCCCG-3′ |
| CS | 5′-CCCGGATCCCAGCGCGGTTTCGCCGCCGC-3′ |
| CA | 5′-CCCTTACGCCTGGGTGCTATCCG-3′ |
| N2/3 | 5′-GGATCCCTGTTCCGGGCCACGACGGC-3′ |
| C2/3 | 5′-GGATCCACCCCGAAAGATCATATTGG-3′ |
S1, S2, S3, S4, A1, A2, A3, and A4: primer set of the 1st PCR for the N-terminal cDNA fragment of SARS CoV N protein from the 1st to 220th amino acid residues. S5, S6, S7, S8, A5, A6, A7, and A8: primer set of the 1st PCR for the C-terminal cDNA fragment of SARS CoV N protein from the 215th to 422nd amino acid residues. NS and NA: primer set of the 2nd PCR for the N-terminal cDNA fragment of SARS CoV N protein from 1st to 220th amino acid residues. CS and CA: primer set of the 2nd PCR for the C-terminal cDNA fragment of SARS CoV N protein from the 215th to 422nd amino acid residues. NS and CA: primer set of PCR for the full-length cDNA fragment of SARS CoV N protein from the 1st to 422nd amino acid residues. NS and N2/3: primer set of PCR for the N-terminal cDNA fragment of SARS CoV N protein from the 1st to 282nd amino acid residues. N2/3 and CA: primer set of PCR for the C-terminal cDNA fragment of SARS CoV N protein from the 143rd to 422nd amino acid residues.