FIG. 1.
Detection and quantification of NV genogroup I using the LUX real-time PCR assay. The plasmid standards were diluted in 1:10 steps from 2 × 107 to 2 × 101. (A) Amplification plot of fluorescence intensities (ΔRn) versus the PCR cycle numbers. (B) First-derivative dissociation curves with melting temperatures of the amplicons. (C) Relationship of known numbers of plasmid standards to the threshold cycle (CT), with standard derivations calculated from three independent real-time PCR experiments.