TABLE 1.

Primers used to amplify portions of housekeeping genes and other loci in Brucella

Gene	Forward primer (5′-3′)	Reverse primer (5′-3′)	Fragment size (bp)
abc	TAGGCCGAATAATTGCCTTC	GACCGCTACAACGAGCTGAT	419
aroE	ATGGAAGGCAAGATCGTCAA	CTGGCACAGTTCGTCAACAG	498
cysW	CTTCCCTGCACTTCCATCAG	ACCAATCTCATCCAGGCAAG	546
gdh	GATGGTGTCGGTTTTGTGC	GATATGCTGGTGCATTGTGG	557
groEL	CTGGACGACAGCTTCGAGA	GGCTTCCAAGACCAACGATA	485
pip	CGGTCAGGCGCTTGTAAT	CGCATTCATGTCGAGCAAT	484
omp25	TGGTGGCTATACCGGTCTTT	AGGATGTTGTCCGTCAGCTT	384
omp25	AAGTCAAGCAGGGCTTTGAA	ACCGGATGCCTGAAATCCTTa	395

^aSame primer designated as 25B and used by Cloeckaert et al. (9). A portion of the omp₂₅ gene was sequenced with two overlapping primer sets. The fragment size is approximate due to a tandem repeat at the 3′ end of the sequence.