Table 1.
Association of cullins with pVHL/elongin BC
| Strain | AD-plasmid | BD-plasmid | His | β-Gal |
|---|---|---|---|---|
| CG1945(BC) | (VHL) | (CUL-2) | + | + |
| CG1945(BC) | (CUL-2) | (VHL) | + | + |
| CG1945(BC) | (VHL) | (VHL) | − | − |
| CG1945(BC) | (CUL-2) | (CUL-2) | − | − |
| CG1945(BC) | (VHL) | (CeCUL-2) | + | + |
| CG1945(BC) | (CUL-2) | (A) | − | − |
| CG1945(BC) | (A) | (CUL-2) | − | − |
| CG1945(BC) | (VHL) | (CUL-1) | − | − |
Characterization of associations of various cullins with pVHL in the presence of elongins B and C in yeast. Various pAS2-1 and pACT2 constructs were cotransformed into CG1945-BC yeast and growth on SD+CSM−Ade−Leu−Trp−His and β-gal activity were measured. Expression of proteins was verified by Western blot analysis (data not shown).