Table 2.
Association of hCUL-2, pVHL, and elongin BC
| Strain | AD-plasmid | BD-plasmid | His | β-Gal |
|---|---|---|---|---|
| CG1945 | (VHL) | (B) | − | − |
| CG1945 | (VHL) | (C) | − | − |
| CG1945 | (VHL) | (CUL-2) | − | − |
| CG1945 | (VHL) | (VHL) | − | − |
| CG1945 | (CUL-2) | (B) | − | − |
| CG1945 | (CUL-2) | (C) | − | − |
| CG1945 | (CUL-2) | (CUL-2) | − | − |
| CG1945 | (CUL-2) | (VHL) | − | − |
| CG1945 | (B) | (B) | − | − |
| CG1945 | (B) | (C) | + | + |
| CG1945 | (B) | (CUL-2) | − | − |
| CG1945 | (B) | (VHL) | − | − |
| CG1945 | (C) | (B) | + | + |
| CG1945 | (C) | (C) | − | − |
| CG1945 | (C) | (VHL) | − | − |
| CG1945 | (C) | (CUL-2) | − | − |
| CG1945 | (A) | (B) | − | − |
| CG1945 | (A) | (C) | + | + |
| CG1945 | (B) | (A) | − | − |
| CG1945 | (C) | (A) | + | + |
| CG1945(B) | (VHL) | (C) | + | + |
| CG1945(B) | (VHL) | (CUL-2) | − | − |
| CG1945(B) | (VHL) | (VHL) | − | − |
| CG1945(B) | (CUL-2) | (C) | + | + |
| CG1945(B) | (CUL-2) | (CUL-2) | − | − |
| CG1945(B) | (CUL-2) | (VHL) | − | − |
| CG1945(B) | (C) | (CUL-2) | − | − |
| CG1945(B) | (C) | (VHL) | + | + |
| CG1945(B) | (A) | (C) | + | + |
| CG1945(B) | (C) | (A) | + | + |
| CG1945(B) | (A) | (CUL-2) | − | − |
| CG1945(B) | (CUL-2) | (A) | − | − |
| CG1945(C) | (VHL) | (B) | + | + |
| CG1945(C) | (VHL) | (CUL-2) | − | − |
| CG1945(C) | (VHL) | (VHL) | − | − |
| CG1945(C) | (CUL-2) | (B) | − | − |
| CG1945(C) | (CUL-2) | (CUL-2) | − | − |
| CG1945(C) | (CUL-2) | (VHL) | − | − |
| CG1945(C) | (B) | (CUL-2) | − | − |
| CG1945(C) | (B) | (VHL) | + | + |
| CG1945(C) | (A) | (B) | − | − |
| CG1945(C) | (B) | (A) | − | − |
| CG1945(C) | (A) | (CUL-2) | − | − |
| CG1945(C) | (CUL-2) | (A) | − | − |
Characterization of associations between hCUL-2, pVHL, and elongins B and C in yeast. Various pAS2-1 and pACT2 constructs were cotransformed into CG1945 (top section), CG1945-B (middle section), and CG1945-C (bottom section) yeast, and growth on SD + CSM−Ade−Leu−Trp−His and β-gal activity were measured. Expression of proteins was verified by Western blot analysis (data not shown).