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. 2007 Nov 7;46(1):255–264. doi: 10.1128/JCM.01721-07

FIG. 1.

FIG. 1.

Growth and fluorescence of C. gattii on m-DTDP agar. Row I, plate 1 shows typical growth and a small amount of pigmentation of C. gattii on m-DTDP agar (B and C) on day 2, whereas C. neoformans (A and D) fail to grow. Plate 2 illustrates weak fluorescence (365 nm) on day 2. Plate 3 shows the beginning of strong pigmentation on day 3 for C. gattii (B and C). Plate 4 illustrates the fluorescence observed on day 3 under a Woods lamp (365 nm.). Row II, plate 1 shows typical growth and dark-brown pigmentation of C. gattii on m-DTDP agar (B and C) after 5 to 7 days. In contrast, C. neoformans (A and D) cannot use the d-amino acids and fails to grow. Plate 2 illustrates the absence of fluorescence of C. gattii after the formation of the dark-brown pigments (masks fluorescence). Plates 3 and 4 show the less-typical pattern observed for C. gattii, quad B (top), which produced smaller amounts of the brown pigments with the fluorescence intensity remaining strong when illuminated with a Woods lamp (365 nm).